O'Brien said his side was ready to meet the unbeaten Lakers, after knocking off reigning Haisman Shield premier Numurkah last Saturday.
“It was a really good win, it's always nice to come away with a win against quality opponents,” O'Brien said.
“It's been a good week on the track, everyone is feeling good, but we understand there's more work to do.”
The Cats are back at home and welcome the second-placed Lakers in what shapes to be a quality contest between the bat and ball.
Nagambie boasts two of the top three batsmen in the competition, with brother duo Mark and Luke Nolan starring each week.
Mark leads the competition for runs this season with 286 and has a remarkable average of 95.33 from five innings.
His brother Luke might not be capturing headlines like the Nagambie captain, but he is third for runs scored with 206.
O'Brien said his side's bowling stocks would need to "hit the right areas" if they get a chance to bowl to reigning Lightfoot medallist Mark Nolan.
“Mark is a fantastic batsman and he looks to be in some great form,” he said.
“We know that we'll have to bowl well and make sure that we hit the right areas to limit his impact.”
However, the Haisman Shield's leading wicket-taker is set to be unleashed on the Lakers’ dynamic duo, with Mooroopna's English import Henry Barrow set to lead the way again.
The opening seamer enters tomorrow's contest with 13 wickets, following his five-wicket haul against Numurkah.
Barrow has become a valuable asset to the Cats this season and has terrorised batsmen in his four matches, boasting an impressive strike-rate of 15.69.
Meanwhile, Nagambie's Nathan Fothergill looks to be back bowling at his best after the seamer grabbed 4-15 in his second match of the season, against Waaia last round.
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Katandra v Shepparton United