Police said it was understood two motorcycles, both travelling north on Euroa-Mansfield Rd at Euroa, collided about 4.25pm on Sunday, December 24.
Police said the rider of the first motorcycle, a 77-year-old Lemnos man, received life-threatening injuries and was flown to hospital, but later died.
His pillion passenger, a teenage girl, received non-life-threatening injuries.
The rider of the second motorcycle, a 34-year-old Kialla man, was treated at the scene for minor injuries.
The passenger of this bike, a teenage girl, was uninjured.
The Lemnos man is the 41st life lost on roads within a 50-minute drive of the Shepparton central business district since New Year’s Eve.
He is also the third person to die in crashes on Euroa-Mansfield Rd since July.
An 18-year-old Shepparton woman died in a crash at Euroa on November 3.
A 59-year-old Shepparton man died after his ute left the road and went down a 10m embankment at Gooram on Sunday, July 9.
The speed limit was reduced on a 4.9km section of the road at Gooram, where the July fatality occurred, in December, but this crash did not occur in that section of the road.
Anyone who witnessed this crash or has dashcam footage or information is urged to contact Crime Stoppers on 1800 333 000 or online at www.crimestoppersvic.com.au
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