David Littleproud next Nationals leader

Three people each had nominated for the leader and deputy positions. 

Addressing the media post-spill, Mr Littleproud described his win as "the proudest day of my professional life". 

"I believe passionately in the National Party because we are all there is about regional and rural Australia. We are the conscience of rural and regional Australia right here in this parliament," he said. 

"The National Party today starts its journey towards 2025, with a vibrant team, ready to articulate the policies that are important to regional and rural Australia. 

"But also to draw on the experience of two former deputy prime ministers in Barnaby Joyce and Michael McCormack. To build that bridge of unity and purpose, to make sure that regional and rural Australia isn't forgotten here." 

Mr Littleproud said the Nationals would draw upon the "strength" of Ms Davey and Senate leader Bridget McKenzie in his leadership team. 

"This is about us as a party moving forward, not lurching to the left not lurching to the right but bringing this thing called common sense to Canberra," he said. 

"And I'm proud to say that part of my leadership is two bright, articulate, powerful women. That's the National Party way."

Ahead of the party room meeting in Canberra, Mr Joyce was "sanguine" about retaining his leadership of the Nationals despite facing a challenge.

Mr Joyce made his case on the Seven Network, saying the junior coalition party must have been doing something right given it retained all its seats at the federal election.

"We won every seat we had before the election, we had three retiring members and still won the seats (and) we were in striking distance of one of the two seats we will be able to take in the next election," he said.

"The Liberals lost 19 seats. 

"In the last two elections, that I've been the leader, we've only picked up seats. We must be doing something right but the job is not over."

However Mr Joyce was accused of having Liberal blood on his hands after his party's support of coal turned inner-city voters off sitting moderates.

Former moderate Liberal MP Trent Zimmerman, who lost his North Sydney seat after a 12.5 per cent swing against him, accused Nationals MPs of derailing the campaign for inner-city seats.

"There's a whole package of reasons we lost in inner-city seats like mine. Clearly, climate change was one of the key issues," he told the ABC.

Nationals senator Matt Canavan's strong support of coal mining and his opposition to the coalition policy of net-zero emissions by 2050 impacted the inner-city vote, Mr Zimmerman added.

"I thought Matt Canavan's intervention was one of the killer moments for us.

"There was just an underlying suspicion that at the end of the day that people like Canavan .. and Barnaby Joyce would prevail if we were elected."

Mr Zimmerman called on the coalition to recognise Labor's policy of a 43 per cent reduction in emissions by 2030, which was significantly higher than the 26 to 28 per cent target the coalition took to the election.