Speaker Milton Dick issued a blistering statement to the house on Wednesday morning after MPs pushed their way out of the chamber during a vote.
The incident happened after the Speaker had directed attendants to lock the doors of the chamber to allow a vote on Tuesday afternoon.
But after he had issued the direction, several MPs continued to make their way out of the chamber.
The Speaker said MPs pushed their way past an attendant which caused them to get stuck in the doorframe and injure their arm.
"I'm particularly disgusted by this behaviour and I will not tolerate it," Mr Dick said.
"For a staff member of this place to be treated in this way, when they are simply doing their job, is disrespectful and a very serious matter."
Frontbench coalition MPs Angus Taylor, Dan Tehan, Andrew Hastie and Ted O'Brien fronted the house to apologise for leaving the chamber after the Speaker's directive.
Backbenchers Llew O'Brien, Zoe McKenzie and Sam Birrell also offered their apologies.
Independent MP Helen Haines said the incident was unparliamentary and dangerous and fell below the standards of the codes of conducts MPs had endorsed.
The Speaker has asked parliamentary departments to launch an urgent risk assessment to ensure there are practices in place to keep staff safe.
Mr Dick said Australians expected their elected representatives to maintain the highest standards of conduct and behaviour.
He understood MPs had full schedules, but no one was too busy to ensure the safety of staff.
"I'm committed to ensuring that this building and this chamber is a safe and respectful place of work for all," he said.