Bowers fronted the AFLW Tribunal on Thursday night to challenge the ban she received for a dangerous off-the-ball bump.
Bulldogs midfielder Kirsty Lamb was tracking her opponent about 40 metres off the ball when Bowers came in with a forceful hip-and-shoulder during Tuesday's match.
The front-on bump caught Lamb unaware and she was left grabbing at her jaw while gingerly leaving the field a short time later.
The incident was assessed as intentional conduct, medium impact and high contact, drawing a two-match ban.
Bowers accepted the contact was medium impact and high contact, but Fremantle's QC Seamus Rafferty looked to argue that the incident should be re-considered from intentional conduct to careless conduct.
However, the Tribunal upheld the original decision of a two-match ban.
It means Bowers will miss Sunday's clash with North Melbourne, and the following match against Carlton.
Bowers shared last year's AFLW best and fairest award with Collingwood skipper Brianna Davey, and she was on track to go back-to-back this season after producing a series of star displays in Fremantle's 5-0 start to 2022.
The Tribunal's decision means she can no longer win the award.
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