The versatile 33-year-old, whose contract with Inter Milan expired at the end of the season, has signed a two-year deal.
Manager Antonio Conte's first major close-season signing highlights a change in policy at the club, who usually sign younger players with re-sale value.
"I'm really excited to start with the Spurs family," Perisic said. "I've really wanted to come here to the Premier League since 2009 when I started in professional football in Belgium, and I can't wait."
The move sees Perisic reunited with Conte who he played under when Inter won the Serie A title in 2020-21.
"I'm looking forward to seeing him again," Perisic said of the Italian. "He lives football, every minute, every hour, every day, even when he's sleeping I think he's thinking about football. I like his character and know he's going to do a great job here."
Perisic, who can play as a left wing-back or even up front, joined Bundesliga club Borussia Dortmund in 2011 where he won the league and German Cup in his first season.
After a move to VfL Wolfsburg he joined Inter in 2015 for whom he played more than 200 Serie A games, scoring 49 goals.
He had a season on loan with Bayern Munich in 2019-20 and won the treble of Bundesliga, Champions League and German Cup. On his return to Inter, under Conte, Perisic won the Serie title and this season helped the club win the Coppa Italia.
Perisic has 113 caps for Croatia and has scored 32 goals including the equaliser against England in the 2018 World Cup semi-final and a goal in the final as Croatia lost to France.
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