The halfback did some running on Thursday away from the cameras but failed to train with the main group ahead of Friday night's sudden-death blockbuster at AAMI Park.
Melbourne are remaining tight lipped on the exact nature of Hughes's injury.
"(Hughes) done a little bit of running this morning, but we'll just see how he pulls up," Storm coach Craig Bellamy said.
"To be honest, he's probably unlikely, but we'll just see how he pulls up this afternoon after the run."
Tyran Wishart could come into the 17 as a replacement for Hughes, or Harry Grant could be an option to switch from hooker and partner with star Cameron Munster in the halves.
"He (Wishart) done most of the training there (on Thursday), but we've also got a couple of other options we can use," Bellamy said.
"There's obviously a bit of shuffling going on.
"Whether the less shuffling, the better, or the more shuffling the better.
"But we've got a plan that hopefully we'll be able to stick to."
Melbourne were humbled 26-0 by Brisbane last Friday in their first loss against the Broncos at Suncorp Stadium since 2009.
Since winning the 2020 premiership in legend Cameron Smith's final game, the Storm are 1-3 in finals, losing their last three dating back to the 2021 preliminary final against Penrith.
On a nightmare night for the Storm up in the Queensland capital, they had to deal with the blow of again losing Ryan Papenhuyzen to a season-ending injury.
"I think everyone's been in touch with him (Papenhuyzen)," Bellamy said.
"It was a bit flattening, to be quite honest, for a couple of days.
"We all feel for him, what he's been through and what he's about to go through again. That hasn't been easy."
Winger Xavier Coates has already been ruled out for the Storm after suffering an ankle injury against the Broncos.
Bellamy has recalled Reimis Smith as Coates' replacement, along with centre Justin Olam.
The pair have been in and out of Bellamy's starting line-up this season.
"They bring a bit of experience in there," Bellamy said.
"It wasn't easy, leaving them out, to be quite honest, because they've done such a good job over a long period of time.
"But I just felt it needed to doing so so we done it.
"But hopefully, they'll be all excited about (playing the Roosters) and I have no doubt their experience will be good for the team."
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