The Broncos coughed up a 14-point half-time lead on Friday night in Sydney and to make matters worse, they lost Ben Hunt to a hamstring injury.
Hunt limped straight up the tunnel after pulling up sore on a kick chase in the second half, with his injury a chance to end Ezra Mam's exile in a matter of days.
Broncos coach Michael Maguire previously refused to put a timeline on Mam's return following his drug-driving ban.
But the coach may have little choice but to recall the five-eighth for next week's clash against St George Illawarra given his replacement's injury.
Hunt's hamstring issue has also thrown his Queensland selection chances into doubt.
After kicking the penalty goal that locked the scores 14-14 with 10 minutes left, Mitchell put Souths on the path to victory by slotting a field goal from 49 metres out with three minutes remaining.
He rubbed salt into the Broncos' wounds by crashing over from dummy half after the siren at a rainy Accor Stadium.
The win breathes new life into a South Sydney campaign decimated by injuries, with the Rabbitohs pushed into the bottom four by a quartet of consecutive losses.
Souths had conceded at least 24 points in each of those losses but had renewed defensive vigour on Friday night.
A lot of that was down to Mitchell, who pulled off two try-saving one-on-one tackles in the backfield.
He first stopped a rampaging Payne Haas on the prop's 40-metre tear down the left side.
Mitchell then dragged Deine Mariner into touch when the winger looked to make it a two-score game in the second half.
Mariner had put the Broncos up by 14 and deflated the Rabbitohs in the minutes before half-time following a shift down the right.
But Souths fired back only minutes after the break when Siliva Havili burrowed past three defenders from dummy half.
The margin was only two points when Isaiah Tass bulldozed through Kotoni Staggs ahead of his try down the left side.
Mitchell slotted a simple penalty goal from directly in front after Xavier Willison hit Lachie Hubner with a high shot, before going on to seal the most unlikely of wins.