Holmes rolled the dice on Tuesday, opting to seek a reduced charge for his grade-two careless high tackle following last weekend's loss to the Gold Coast.
The Australian international would have escaped with a fine but risked incurring an extra game added on to his three-game suspension if he was unsuccessful.
Unfortunately for Holmes the move backfired and he was handed an additional week, meaning he won't return for the Cowboys this season unless they secure themselves a place in the top eight.
Rookie Zac Laybutt looms as the most likely replacement for Saturday's derby with Brisbane and could be in line for more NRL time with Holmes also set to miss their remaining games against Cronulla, the Dolphins and Penrith.
St George Illawarra forward Jack de Belin also failed to get his hip-drop tackle charge downgraded at the judiciary and will miss Sunday's game against Parramatta.
Five-eighth Dylan Brown is available for the Eels after serving a suspension for groping a woman in a Sydney nightclub.
Elsewhere, Melbourne have man mountain Nelson Asofa-Solomona (finger) fit for their trip to Penrith on Friday as the Panthers named Zac Hosking in the centres.
Hosking is filling in for Tyrone Peachey (suspension) although Izack Tago is part of the Panthers' extended squad and could slot into the centres if given the green light.
Storm fullback Ryan Papenhuyzen is due to make his comeback game in the Queensland Cup nearly 13 months after fracturing his kneecap.
Canberra, meanwhile, have dropped hooker Tom Starling from the interchange bench for rookie Adrian Trevilyan for Sunday's meeting with the Wests Tigers.
Winger Nick Cotric comes in for Jarrod Croker (hamstring) with Seb Kris moving to centre and Jordan Rapana assuming fullback duties.
As part of Saturday's double header in Perth, an unchanged Newcastle side face the Dolphins while Cronulla have named Connor Tracey at fullback for their meeting with South Sydney.
In Friday's early kick-off, Jazz Tevaga is in line for his first game since April as the Warriors face the Gold Coast, whose fullback AJ Brimson has been cleared of a rib injury and is good to go.
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