The Heat, who will bowl first at the Gabba, have been strengthened with the return of world No.1 Test batsman Marnus Labuschagne but are out of the finals contention after five-straight losses.
The Sixers, who also welcome back Lyon's fellow spinner Stephen O'Keefe, can effectively snuff out the hopes of cross-town rivals the Sydney Thunder finishing second with a bonus-point win.
Brisbane Heat: Chris Lynn, Max Bryant, Marnus Labuschagne, Ben Duckett, Sam Heazlett, Jimmy Peirson (capt) (wk), James Bazley, Michael Neser, Mark Steketee, Mitch Swepson, Matt Kuhnemann.
Sydney Sixers: Josh Philippe (wk), Jack Edwards, Daniel Hughes, Moises Henriques (capt), Justin Avendano, Dan Christian, Sean Abbott, Hayden Kerr, Ben Dwarshuis, Nathan Lyon, Stephen O'Keefe.
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