Meg Lanning will open the batting for the hosts in place of the injured Alyssa Healy (calf), while Alana King holds her spot as legspinner ahead of Georgia Wareham.
The series marks the last ahead of the Twenty20 World Cup, with Australia facing the reality of a middle-order selection squeeze ahead of the tournament.
Pakistan have gone with three spinners for the match on the postage-stamp size ground.
AUSTRALIA: Beth Mooney, Meg Lanning (capt), Tahlia McGrath, Ashleigh Gardner, Ellyse Perry, Annabel Sutherland, Jess Jonassen, Alana King, Megan Schutt, Darcie Brown.
PAKISTAN: Muneeba Ali, Javeria Khan, Bismah Maroof (capt), Sadaf Shamas, Omaima Sohail, Ayesha Nasem, Fatima Sana, Tuba Hassan, Aiman Anwar, Sadia Iqbal, Nida Dar.
10°C