Tony Elbayeh, 39, and Hysam Hamdan, 43, were extradited from NSW to Queensland on Thursday after being arrested in Sydney in relation to the death of Omega Ruston at Burleigh Heads on January 26, 2009.
Elbayeh is charged with murder, accessory after the fact, corruption of a witness and 30 counts of perjury.
Hamdan, arrested while allegedly attempting to board a flight to Dubai carrying $25,000 cash, faces a murder charge with an alternative of accessory to murder.
The pair did not appear as charges were mentioned in Brisbane Magistrates Court on Thursday before being adjourned for mention again on February 28.
Both men cannot apply for bail and remain in custody.
A third man Paul Younan, 37, who police allege was the gunman in the road rage incident, is yet to appear in a Brisbane court.
Former bikie enforcer Brent Luke Simpson, 45, faced court on Wednesday charged being an accessory, attempting to corrupt a witness and 18 counts of perjury.
Simpson allegedly "provided immediate assistance to the offenders after the murder had been committed".
A bail application for Simpson was adjourned until February 17.
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