Italy were thrashed 73-24 by France in their last Six Nations fixture, which Melbourne's French-based Ioane missed due to a knee injury.
While the 30-year-old Lyon back Ioane returns, Ange Capuozzo will play at full-back while Matt Gallagher, Â a former England Under-20 international and son of New Zealand 1987 World Cup winner John Gallagher, makes his Six Nations debut on the left wing.
Gallagher made his debut for Italy against Samoa in July last year and also played in a 20-17 victory over Georgia in November.
Asked about the changes, Quesada pointed to the team's performance against France, saying: "In the second half we struggled to defend as we wanted to.
"It was not just a technical or organisational problem, rather there was no respect for roles and principles, I had never seen the team lost, trying to solve things individually, without a collective organisation."
In midfield, Quesada has opted to stick with the centre pairing of Juan Ignacio Brex and Tommaso Menoncello, dubbed 'Brexoncello'.
Welsh-born scrumhalf Stephen Varney has been drafted in alongside Paolo Garbisi at number 10, while Simone Ferrari and Gianmarco Lucchesi drop to the bench with Marco Riccioni and Giacomo Nicotera coming into the front row.
Tommaso Allan, the most experienced player in Italy's squad, has been named on the bench.
England are third in the standings on 10 points, six ahead of fifth-placed Italy heading into the penultimate round of matches. The two teams have faced each other 31 times in Tests, all of which have been won by England.
But Italy are looking to improve on their showing in last year's tournament, where they ended with a three-game unbeaten run, recording two wins and a draw, gathering 11 points.
"Now we will have to assess whether it (France defeat) was an accident for a team that has been together for just over a year, and whether we will be able to resume a linear path and show who we really are," Quesada added.
