Demonstrators will meet at the State Library of Victoria at 12pm on Sunday to call for an end to the war in Gaza.
It comes after pro-Palestine and pro-Israel groups faced off in Melbourne on Friday night following a suspicious fire at a nearby burger shop.
Police used pepper spray as a fight broke out in the middle of Hawthorn Road and one person was hit by a rock.
On Friday, Victoria Police Inspector Scott Dwyer said he was confident the fire was not religiously or politically motivated.
However, the owner Hash Tayeh claims he has received threats and the fire was a hate crime sparked by his involvement in a pro-Palestine rally and has agreed to speak at Sunday's event.
Later on Friday pro-Palestine supporters rallied in Princes Park near the burger shop and were greeted on the other side of the road by pro-Israel demonstrators, with officers wedged between the groups.
The clash sparked the evacuation of a nearby synagogue, which the protest organisers have apologised for.
On Saturday, Victoria Police said there would be additional patrols in the Caulfield area and talks were under way with community groups.
"Our priority at these events is to keep the peace and ensure the event is safe for those attending and the broader community," a spokesperson said.
"While individuals have the right to protest lawfully, we strongly encourage those attending to protest peacefully and without impacting the broader community."
The scenes in Caulfield sparked condemnation, including from Prime Minister Anthony Albanese who said all Australians have a responsibility to preserve peace.
"There is no place in our nation for hatred or prejudice of any kind," he said.
Opposition leader Peter Dutton hit out at what he described as "a deliberate act of incitement designed to end in violence" and anti-semitism.
"The behaviour we saw last night in Caulfield by individuals provocatively protesting in an area home to many people of the Jewish faith was absolutely disgusting," he posted on X.
Premier Jacinta Allan said anti-Semitism in every form and attacks on the Islamic community were unacceptable.
"We cannot let violence in the Middle East beget violence on the streets of Melbourne," she told reporters.
Opposition Leader John Pesutto said the clash in Caulfield was "blatantly anti-Semitic" and all Victorians should be free to worship safely without fear of intimidation.
Sunday's rally will also be addressed by Palestinian academic Micaela Sahhar, Greens MP Samantha Shantini Ratnam and others.
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