The working bee saw 10 Friends of the Botanic Gardens volunteers plant about 350 new plants that are under threat in the wild.
The plants will be in garden beds along the pathway towards the river walk, children's garden and weaving garden.
President Jill Grant said the Friends received the plants as part of the Care for the Rare program.
“We put up our hands to do this back in 2017,” she said.
“The plants have been grown for us and we have the first delivery so everyone is keen to watch how our continue to grow.
“Because they're rare and threatened in the wild this will be a source for propagation, as well as seed collecting.
“It's also an indication of what colours you can plant. Let's hope we can get some rain.”
There's about another 300 plants to arrive and planted on a later date.
Mrs Grant said the Australian Botanic Gardens Shepparton was lucky not to be closed during strict COVID-19 conditions.
She said it had been an excellent flowering season because of the rain and the wild flowers were coming through in the natural bushland.
The Care for Rare program was supported by the Helen MacPherson Smith Trust and collaborated through the Botanic Gardens Australia and New Zealand Victoria network.
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