Leo, 71, on Sunday said a small nodule had been found during a check-up, was "well-contained" and had been spotted early.
He was expected to leave the Vatican for Rome's Gemelli hospital on Tuesday, the Vatican press office said, without specifying when the actual operation would take place.
Leo, elected by the world's cardinals in May 2025, is relatively young for a Church leader. He enjoys sports, including tennis, and is not known to be a smoker or to have had health issues previously.
The Vatican has said his recovery is expected to take about a week. All his public events have been cancelled until next Tuesday.
A nodule on the lung is a small, localised lesion that can be benign. The American Thoracic Society says fewer than five per cent of lung nodules overall prove cancerous, although risk rises with age, size, and other factors such as smoking.
Dr. Federico Venuto, chief of thoracic surgery at Rome's Policlinico Umberto I hospital, said procedures to remove lung nodules are usually minimally invasive, and can sometimes involve use of a thin camera tube called a thoracoscope.
"The complication rate is extremely low," he told Reuters.
The late Pope Francis, Leo's predecessor, died aged 88 in April 2025 and was in hospital several times as he grew frail in his later years.
Leo, who spoke in a firm, clear voice at his only public event on Monday, keeps a very busy schedule. He is due to make an 11-day tour of Uruguay, Argentina and Peru from November 6 to 17.
In the same address on Sunday when the pope announced his need for surgery, Leo pledged he would go forward with the trip.
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