The Peter Ross Edwards causeway between Shepparton and Mooroopna was closed to traffic last night.
A female pedestrian was struck by a car around 9pm.
The causeway was closed for up to an hour while paramedics attended to the woman and police investigated the crash.
The woman was transported by road to the Shepparton airport, before being airlifted to Melbourne.
Traffic was diverted along Watt Rd.