Southbound lanes of the Hume Freeway are closed at Violet Town.
A truck rolled between Violet Town and Euroa about 4.30am this morning.
The crash has resulted in a large diesel spill.
Traffic is being diverted via Murchison-Violet Town Rd and the Goulburn Valley Hwy.
Police, Vicroads and heavy haulage are at the scene.
It is unknown when the road will be reopened.